Effects of Sphingosine 1-Phosphate/Sphingosine-1-Phosphate Receptor on Pro-Angiogenesis in HT-29 Human Colon Cancer Cells

To investigate the effects and mechanism of sphingosine-1-phosphate/sphingosine-1-phosphate receptor on pro-angiogenesis in human colon cancer cells is main objective. The sphingosine-1-phosphate HT-29 SW480 were assessed by tube formation assay. Quantitative real-time polymerase chain reaction was used to detect expression interleukin-8, interleukin-6 vascular endothelial growth factor HT29 after treatment. Small interfering ribonucleic acid designed silence sphingosine-1- phosphate 1, 2 3 cells. Western blot quantitative assess down-regulation efficiency Later, detected reaction. Tube assay showed that number formed tubes umbilical vein cell-fused (EA.hy926) resuspended culture supernatant from treated with significantly more than control group (t=3.667 4.881, p=0.021 0.013), indicating promoted pro-angiogenic ability Moreover, messenger increased treatment compared (p<0.05) where 1 gene could decrease (p<0.05), while did not lead significant changes. Sphingosine-1-phosphate up-regulated through thereby enhancing proangiogenic effect pathway a promising novel therapeutic target for inhibiting growth.